Journal: BMC Complementary and Alternative Medicine

Article Title: Supercritical fluid extract of Lycium chinense Miller root inhibition of melanin production and its potential mechanisms of action

doi: 10.1186/1472-6882-14-208

Figure Lengend Snippet: The effects of Lycium chinense Miller root SFE on melanin content in PD98059, SB 203580-, SP600125- and IBMX-treated B16F10 cells. The results are presented as percentages of the control values, and the data are presented as the mean ± S.D. of three separate experiments. The values are significantly different compared with the control. * p < 0.05.

Article Snippet: Protein kinase regulators, including3-isobutyl-1-methyl-xanthine (IBMX), SB203580 (p38 MAPK-inhibitor), SP600125 (c-Jun N-terminal kinase inhibitor; JNK inhibitor) and PD98059 (MEK 1/2-inhibitor), were obtained from Tocris (Ellisville, Missouri, USA).

Techniques: Control

Journal: Viruses

Article Title: Regulation of Stress-Activated Kinases in Response to Tacaribe Virus Infection and Its Implications for Viral Replication

doi: 10.3390/v14092018

Figure Lengend Snippet: Host cell kinase inhibitors and activators used for growth kinetics.

Article Snippet: c-Jun ↓ , c-JUN blocking peptide , Tocris # 1989 , 50 μM.

Techniques: Blocking Assay

Journal: JCI Insight

Article Title: Pentraxin-2 suppresses c-Jun/AP-1 signaling to inhibit progressive fibrotic disease

doi: 10.1172/jci.insight.87446

Figure Lengend Snippet: (A and B) Time-course Western blots (A) and densitometry quantification (B) showing c-Jun phosphorylation (p–S63-c-Jun) and total c-Jun levels from 15 to 60 minutes after rhPTX-2 and/or TNF-α stimulation in human iPSC-derived monocytes, normalized to GAPDH. Note that rhPTX-2 attenuates both total and p–c-Jun in response to TNF-α. Data represent 3 experiments. (C) Effect of concentrations of PTX-2 or AP-1 inhibitor SR11302 on TNF-α–induced AP1/NF-κB promoter activity in human monocytes. (D) Effect of concentrations of PTX-2 on TNF-α–induced AP-1 promoter activity alone, in human monocytes. (E) rhPTX-2–induced changes in the secretion of factors associated with fibrosis by primary human monocytes cultured from 12 to 120 hours. Presence of c-Jun and AP-1 binding sites in the promoters of these genes is indicated, based on the GeneCards database. n = 3–8/group (*P < 0.05, **P < 0.01, ***P < 0.001 (ANOVA with post hoc testing for multiple comparisons). VEH, vehicle; AlkPhos, alkaline phosphatase.

Article Snippet: The c-Jun inhibitor SR11302 (Tocris Bioscience) was tested for toxicity in THP-1 cells and used at a dose at least 5-fold below the detection of decreased viability.

Techniques: Western Blot, Phospho-proteomics, Derivative Assay, Activity Assay, Cell Culture, Binding Assay